Lab+Protocols

Booking Calendars (JTM and JFK Labs):   Link to Shaker Booking Link to CFX-96/PCR Booking  Link to Superdex Booking  Link to Small Superdex Booking  Link to Other Columns Booking  Link to FPLC Booking

Lab Protocols


 * Gateway System:**

(1) pDONR Vectors

(2) DB 3.1 Cells


 * Recombinant Protein Expression:**

(1) Hunt 2005 - an excellent review on the concepts of recombinant protein expression including bacteria, insect and mammalian

(2) Autoinduction protein expression


 * Cell Culture Guidelines:**


 * Mitochondrial Imaging:**


 * Vectors:**

Cloning Vectors:

Gateway DONR Vector:

Gateway Destination Vectors:

pET Vectors:


 * Standards:**

DNA Ladders:

400 uL protein solution + 100 uL BioRad Assay Mix (neat)
 * BioRad MicroAssay Standard Curve:**


 * Real time PCR:**

Overview


 * Circular Dichroism:**

CDPro Website DichroWeb


 * Enzyme Kinetics:**